Protein Services
rewards center
24 hour
You are here: Biology CRO for Drug Discovery »    Protein Services » Bacterial Expression » Guaranteed Package

E.coli Expression—SC1253 Guaranteed Package ($2,200)

Milestones Specifications Deliverables Timeline1
1. Subcloning
  • Subclone the gene into 3 expression vectors (Customer provides the gene or GenScript synthesizes the gene for an additional fee)
Construct(s) report
2 weeks
2. Expression optimization and verification
  • Transformation of 3 constructs into bacteria
  • Expression optimization: induction time, temperature, and IPTG concentration
  • Coomassie blue stained SDS-PAGE gel
  • Yield estimation and feasibility proposal for large-scale expression (optional)
QC data
1–2 weeks
3. Expression and purification
  • Expression in 1 L bacterial culture
  • One-step affinity purification
  • QC by SDS-PAGE, Western blot2, and MALDI-TOF3
3 mg or more of purified soluble tagged protein
QC data
1–2 weeks

1 Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.

2 FREE Western QC data is available for tagged proteins that can be detected by the anti-His or anti-GST antibody. For other tagged proteins, additional charges may apply.

3 Optional MALDI-TOF data is available with additional charge.

Quotations and Ordering:

protein services To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

protein services To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.

protein services If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.

protein services For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure online messaging system.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Order by mail Email: protein@genscript.com
Order by phone Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Order by fax Fax: 1-732-210-0262 1-732-885-5878
e.coli expression quotation
Related Service & Products
Promotion