ONE-HOUR IP-Western Kits
IP-Western analysis frequently suffers from heavy/light chain, protein A and G contamination, particularly when the molecular weight of the target protein is close to those of the contaminants. This problem can now be solved using GenScript ONE-HOUR IP-Western Kits. This breakthrough in Western technology has several significant advantages: enhanced sensitivity, elimination of background noise and increased chances of identifying rare proteins. Like other ONE-HOUR Western™ Kits, the ONE-HOUR IP-Western Kits also reduce total western analysis time to about one hour. |
Key Features:
- No Contamination: GenScript proprietary blocking reagents eliminate all contamination originated from antibody heavy/light chains, and proteins A and G.
- Superior Sensitivity: Comparable to or better than that of classical three-step Western blot kits. Taking the reduced background into account, the effective sensitivity of the ONE-HOUR IP-Western kit is even higher.
- Simple Procedure: Fewer and simpler steps than classical Western blot kits, leaving less room for human error.
- No Additional Secondary Antibody Required: ONE-HOUR IP-Western Kits include GenScript proprietary secondary antibodies, eliminating the need for additional secondary antibodies.
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Applications:
ONE-HOUR IP-Western Kits enable the fast Western-blot for detecting proteins from immunoprecipitation using:
- Protein A, G or A/G MagBeads
- Secondary Antibody Coated MagBeads
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Examples:
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A Trx fusion protein was immunoprecipitated using anti-Trx (GenScript, A00180) mouse monoclonal antibody bound to Protein A MagBeads (GenScript, L00273).Trx fusion protein was then immunoblotted with the same primary antibody by using HRP-conjugated goat anti-mouse IgG (Fc specific, Sigma, A 0168) (Lane 1) and using the ONE-HOUR IP-Western Kit L00232 (Lane 2). |
Ordering Information:
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