The Problem: Contamination from heavy chains, light chains, and proteins A and G can render western analysis impractical for analyzing IP results, particularly those involving proteins whose molecular weights match those of the contaminants.
Our Solution: GenScript's ONE-HOUR IP-Western kit not only trims IP-western analysis time to one hour but also completely eliminates all contamination from heavy chains, light chains, and proteins A and G.
Our Strategy: Our ONE-HOUR IP-Western Kit is the only product on the market that solves both the major problems of IP-western analysis, providing a quick and simple procedure to go with its uncontaminated results.
Other features of these kits include the following:
Speed: One-hour run time
Clarity: Ultra-low background, journal-quality images
Flexibility: Available with mouse, rabbit, and goat primary antibody
Read more about ONE-HOURTM IP-Western Kit
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Figure 1:
A Trx fusion protein was immunoprecipitated using anti-Trx (GenScript, A00180) mouse monoclonal antibody bound to Protein G Resin (GenScript, L00209).Trx fusion protein was then immunoblotted with the same primary antibody by using HRP-conjugated goat anti-mouse IgG (Fc specific, Sigma, A 0168) (Lane 1) and using the ONE-HOUR IP-Western Kit L00232 (Lane 2). |
