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TissueDirect™ Multiplex PCR System

*This product has been discontinued!*
DNA Preparation/PCR System from Tissues
L00194
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Description


Description:
TissueDirectTM Multiplex PCR System is a powerful reagent kit for both easy and rapid genomic DNA preparation and multiplex PCR amplification. Genomic DNA is directly released from cells (tissues, mouse tails, hair shafts, and cell culture) using proprietary reagents in 12 minutes without DNA purification. The genomic DNA can be used immediately in PCR amplification of multiple gene targets (up to >1,000) or stored at 4°C for future use (stable at least 6 months at 4°C).
Product Introduction
Key Features
Key Features:
  • Easy to perform: Our simple, speedy procedure extracts DNA in 12 minutes.

  • High specificity: “HotStart” ScriptTM DNA Polymerase (a GenScript proprietary DNA polymerase), yields highly specific amplification results.

  • Excellent sensitivity: Genomic DNA from a single sperm has been successfully used in multiplex PCR amplification of more than 1000 amplicons and subsequent DNA genotyping assays. TissueDirect can work with even the smallest amounts of tissue, preserving precious samples and permitting less invasive methods.
Storage Store at -20°C
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Applications
Applications:

The TissueDirectTM PCR System can extract genomic DNA from the following types of samples:

  • Tissues (fresh, frozen or paraffin), tissue microdissections
  • Animal cells (such as those from mouse tails)
  • Yeast cells
  • Buccal cells
  • Cell cultures
  • Hair shafts
  • Saliva
  • Sperm

    The resulting genomic DNA is suitable for applications such as the following:
    • SNP genotyping and mutation detection
    • Target detection in transgenic mice
    • DNA sequencing and cloning
    • Quantitative PCR

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