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Name
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L00208
Protein Expression and Purification Kit
1 kit
$335.00

Protein Expression and Purification Kit



Description:

GenScript Protein Expression and Purification Kit (L00208) provides a set of reagents for researchers to perform the expression and purification of target proteins. pGS-21a vector is designed for cloning, high-level expression, and the convenient purification of proteins. Either of two tags, (His)6 and GST, may be joined to the N- terminus of the fused protein to facilitate its purification. Recombinant enterokinase light chain (EK) is also included in the kit for the cleavage of tags. The recombinant enterokinase (EK) contains a (His)6 tag for easy removal of EK after cleavage using Ni-resin (not included).

Key Features:
  • Easy to perform: Simple and fast procedure for purification of GST-fusion proteins.
  • High capacity: The system can support over 6 mg horse liver GST/ml medium.
  • Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.
Examples:



Fig.1. Purification profile using GenScript Glutathione Resin. Whole proteins (Lane 1) were passed through GST-resin. The flow-through (Lane 2) contains most of the whole proteins. After wash (Lane 3), GST protein (Lane 4) was eluted.









Components
Components  L00208  L00207  L00206 
Columns  5 empty columns  5 empty columns   
Enterokinase  2 X 100 IU     
Expression Vector  pGS-21a     
Glutathione Resin  10 ml (20 ml of 50% slurry )  10 ml (20 ml of 50% slurry )  10 ml (20 ml of 50% slurry) 
Glutathione, reduced  5 X 0.154 g  5 X 0.154 g   


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