Protein Expression and Purification Kit

Proteins, Expression, Isolation and Analysis

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Protein Expression and Purification Kit

Description:

GenScript Protein Expression and Purification Kit (L00208) provides a set of reagents for researchers to perform the expression and purification of target proteins. pGS-21a vector is designed for cloning, high-level expression, and the convenient purification of proteins. Either of two tags, (His)6 and GST, may be joined to the N- terminus of the fused protein to facilitate its purification. Recombinant enterokinase light chain (EK) is also included in the kit for the cleavage of tags. The recombinant enterokinase (EK) contains a (His)6 tag for easy removal of EK after cleavage using Ni-resin (not included).

Key Features:

Easy to perform: Simple and fast procedure for purification of GST-fusion proteins.
High capacity: The system can support over 6 mg horse liver GST/ml medium.
Stability: This reusable resin shows no obvious decrease of the binding capacity after three uses.

Examples:

Fig.1. Purification profile using GenScript Glutathione Resin. Whole proteins (Lane 1) were passed through GST-resin. The flow-through (Lane 2) contains most of the whole proteins. After wash (Lane 3), GST protein (Lane 4) was eluted.

Components

Components	L00208	L00207	L00206
Columns	5 empty columns	5 empty columns
Enterokinase	2 X 100 IU
Expression Vector	pGS-21a
Glutathione Resin	10 ml (20 ml of 50% slurry )	10 ml (20 ml of 50% slurry )	10 ml (20 ml of 50% slurry)
Glutathione, reduced	5 X 0.154 g	5 X 0.154 g

Document

TECHNICAL MANUAL: 20100420070236 (PDF)
MSDS: 20100420070431 (PDF)

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