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ChromoSensorTM One-Solution TMB Substrate


Tetramethyl benzidine (TMB), a noncarcinogenic analog of benzidine, is routinely used as the key substrate for enzyme immunoassays involving horseradish peroxidase (HRP)-labelled antibodies and molecular probes.

GenScript ChromoSensorTM One Solution TMB Substrate is a specially formulated, ready-to-use, single-solution substrate designed for ultra-sensitive detection of horseradish peroxidase in Western blot or Dot blot assays.

Key Features:
  • Easy to perform: The kit contains a ready-to-use, single-solution substrate.
  • High sensitivity and low background: The substrate is optimized to give high signal and low background.
  • Reproducible results: The substrate produces highly reproducible results.

Shown below is a Western blot detection of housekeeping protein α-Tubulin.
Figure 1. Western blot detection of α-Tubulin using the ONE-HOUR WesternTM Blot Kit (GenScript, L00205) and monoclonal α-Tubulin antibody (abcam, ab7291). 10 µg, 5.0 µg, 2.5 µg, 1.3 µg, 0.63 µg, and 0.32 µg of Hela cell lysate (BD Biosciences, 611449) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, and Lane 6, respectively. The blot was developed with ChromoSensorTM One-Solution TMB Substrate.

Examples of using ONE-HOUR Western<sup>TM</sup> GST Blot Kit


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