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L00296
 TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)
1 kit
$598.00

TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)

Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (Biotin-labeled POD) (Cat. No.L00296) is one of GenScrip’s newly introduced products. It is used for detecting the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3,3’-Diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Key Feature
  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
    • Technical Manual:

    Components
    Components  L00296 
    DAB  10 mg 
    DNase I  1ml (50000U) 
    DNase I buffer  1ml 
    Equilibration Buffer  5.0 ml 
    Streptavidin-HRP  50 μl 
    TdT  400 μl 
    Biotin-11-dUTP  100 μl 


    Document


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