TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)

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TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)

Description

The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (Biotin labeled POD ) (Cat. No. L00297) is one of GenScript's newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Key features

Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.

Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.

Enhanced Specificity: The kit can stain apoptotic cells.

Streamlined Process: The entire procedure takes about three hours.

Increased Convenience: The results can be observed by light microscope.

Technical Manual:

Download PDF file TM0263

Components

Components	L00297
DAB	10 mg
Equilibration Buffer	5.0 ml
FITC-12-dUTP
Streptavidin-HRP	50 μl
TdT	400 μl
50X Proteinase K	200 μl
Anti-fluorescein Antibody
Biotin-11-dUTP	100 μl

Document

MSDS: 20090218074115 (PDF)

Related Products

Cat. No.	Name	Size	Price
L00290	TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)	1 kit	$598.00
L00296	TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)	1 kit	$598.00
L00299	TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)	1 kit	$598.00
L00300	TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)	1 kit	$598.00
L00301	TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)	1 kit	$598.00


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