*This product has been discontinued! *
TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD)
Description
TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD ) (Cat. No. L00298) is one of GenScript's newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3′-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features
Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
Enhanced Specificity: The kit can stain apoptotic cells.
Streamlined Process: The entire procedure takes about three hours.
Increased Convenience: The results can be observed by light microscope.
Technical Manual:
Components:
|
Components
|
L00300 |
L00299 |
L00290 |
L00296 |
L00297 |
L00301 |
|---|
|
DAB
|
10 mg |
10 mg |
10 mg |
10 mg |
10 mg |
10 mg |
|---|
|
DNase I
|
1ml (50000U) |
|
1ml (50000U) |
|
|
|
|---|
|
DNase I buffer
|
1ml |
|
1ml |
|
|
|
|---|
|
Equilibration Buffer
|
5.0 ml |
5.0 ml |
5.0 ml |
5.0 ml |
5.0 ml |
5.0 ml |
|---|
|
FITC-12-dUTP
|
100 μl |
100 μl |
|
|
|
100 μl |
|---|
|
HRP-labeled Anti-FITC Antibody
|
1000 μl |
1000 μl |
|
|
|
1000 μl |
|---|
|
Streptavidin-HRP
|
|
|
50 μl |
50 μl |
50 μl |
|
|---|
|
TdT
|
400 μl |
400 μl |
400 μl |
400 μl |
400 μl |
400 μl |
|---|
|
50X Proteinase K
|
200 μl |
|
200 μl |
|
200 μl |
|
|---|
|
Anti-fluorescein Antibody
|
|
|
|
|
|
|
|---|
|
Biotin-11-dUTP
|
|
|
100 μl |
100 μl |
100 μl |
|
|---|
Document:
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1 |
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2 |
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