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L00298
 TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Sections, Biotin labeled POD)
100 Assays
$598.00

TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Sections, Biotin labeled POD)


Description

The TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD) (Cat. No. L00298) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key Feature
  • Simplified Procedure: The kit contains ready-to-use reagents, including DAB-Solution.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
    Technical Manual:

    Components
    Components  L00296  L00298 
    DAB-A Solution  600μL (for 100 assays)  600μL (for 100 assays) 
    DAB-B Solution  600μL (for 100 assays)  600μL (for 100 assays) 
    DAB-C Solution  600μL (for 100 assays)  600μL (for 100 assays) 
    DNase I (50 U/μL)  1ml (for 100 assays)  1ml (for 100 assays) 
    DNase I buffer  1ml (for 100 assays)  1ml (for 100 assays) 
    Equilibration Buffer  5.0 ml (for 100 assays)  5.0 ml (for 100 assays) 
    Streptavidin-HRP  50 μl (for 100 assays)  50 µl (for 100 assays) 
    TdT  400 μl (for 100 assays)  400 μl (for 100 assays) 
    Biotin-11-dUTP  100 μl (for 100 assays)  100 μl (for 100 assays) 


    Document


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