TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD)

You are here: Products » Cellular Analysis » Apoptosis Detection Kits » TUNEL Detection Kits » Kit : TUNEL Apoptosis Detection Kit (Cryopreserved Tissue Section, Biotin-labeled POD)

*This product has been discontinued! *

TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD)

Description

TUNEL Apoptosis Detection Kit for Cryopreserved Tissue Sections (Biotin labeled POD ) (Cat. No. L00298) is one of GenScript's newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3′-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Key features

Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.

Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.

Enhanced Specificity: The kit can stain apoptotic cells.

Streamlined Process: The entire procedure takes about three hours.

Increased Convenience: The results can be observed by light microscope.

Technical Manual:

Download PDF file TM0264

Document

MSDS: 20090218074459 (PDF)

Related Products

Cat. No.	Name	Size	Price
L00290	TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)	1 kit	$598.00
L00296	TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)	1 kit	$598.00
L00297	TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)	1 kit	$598.00
L00299	TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)	1 kit	$598.00
L00300	TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)	1 kit	$598.00
L00301	TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)	1 kit	$598.00


1	Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2	Bacterial Expression System: GenScript has developed BacPower^TM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3	Yeast Expression System: GenScript's proprietary YeastHIGH^TM technology combines the advantages of the bacterial and eukaryotic expression systems.
4	Baculovirus/Insect Cell Expression System: GenScript developed BacuVance^TM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5	Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.