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L00299
 TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)
1 kit
$598.00

TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)

Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (FITC-labeled POD) (Cat. No. L00299) is one of GenScript's newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, fluorescein-labeled nucleotides bind with the DNA 3'-OH ends using natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). The fluorescence could be observed by fluorescence microscope. And then the compound of anti-fluorescein antibody and HRP is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by fluorescence microscope and light microscope.
    Technical Manual:

    Components
    Components  L00299 
    DAB  10 mg 
    Equilibration Buffer  5.0 ml 
    FITC-12-dUTP  100 μl 
    HRP-labeled Anti-FITC Antibody  1000 μl 
    Streptavidin-HRP   
    TdT  400 μl 
    50X Proteinase K   
    Biotin-11-dUTP   


    Document


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