Double Stain Apoptosis Detection Kit (Hoechst 33342/PI)

*This product has been discontinued!*

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Cat. No.	L00309
Size	Buy in bulk

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Product Introduction

Description

The GenScript Double Stain Apoptosis Detection Kit (Hoechst 33342/PI) provides a rapid and convenient assay for apoptosis based upon fluorescent detection of compacted chromatin in apoptotic cells. This kit contains two ready-to-use dyes bound to DNA. Hoechst 33342, a type of blue-fluorescence dye (excitation/emission maxima ≈350/461 nm when bound to DNA), stains the condensed chromatin in apoptotic cells more brightly than normal chromatin. Propidium iodide (PI), a red-fluorescence dye (excitation/emission maxima ≈535/617 nm when bound to DNA), is only permeant to dead cells. The staining pattern resulting from the simultaneous use of these dyes makes it possible to distinguish normal, apoptotic, and dead cell populations by flow cytometry and fluorescence microscopy.

Product Introduction

Key Features

Key Features

Simplified Procedure: The kit is quick and easy to use.
Fast and Quick: all of the procedure is in 20 minutes.
Strong Versatility: This product directly analyzes by normal, apoptotic, and dead cells by flow cytometry and fluorescence microscopy.

Storage

This kit remains stable for at least one year if stored at 4°C and protected from light.

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Citations

1. Karia P, et al. Breast cancer amelioration by Butea monosperma in-vitro and in-vivo. J Ethnopharmacol. (2018)
2. Chen C, et al. Compression regulates gene expression of chondrocytes through HDAC4 nuclear relocation via PP2A-depended HDAC4 dephosphorylation. Biochim Biophys Acta. (2016-07)
3. Chen C, et al. Cyclic Equibiaxial Tensile Strain Alters Gene Expression of Chondrocytes via Histone Deacetylase 4 Shuttling. PLoS One. (2016-05)
4. Rosenberg N, et al. In vitro catabolic effect of protoporphyrin IX in human osteoblast-like cells: possible role of the 18 kDa mitochondrial translocator protein. J Bioenerg Biomembr. (2013)