PCR and Cloning
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L00339
24 rxns
$175

CloneEZ® PCR Cloning Kit


Description
The GenScript CloneEZ® PCR Cloning Kit is designed for the quick PCR cloning , it is especially powerful in high-throughput cloning of PCR products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases. Using our proprietary CloneEZ® Enzyme, this kit is able to rapidly generate precise and directional constructs by carrying out 30-minute incubation at room temperature (20 °C-25 °C).

Key Features
The CloneEZ® PCR Cloning Kit offers flexible and quick directional cloning and enables users to::
  • Clone into any vector without any need for restriction enzymes, phosphatase treatments, or ligases
  • Provide up to 12 kb PCR cloning at any restriction site
  • Rapidly and precisely generate correctly oriented constructs and inserts
  • Work with PCR that employ any thermostable polymerases


    • Applications
    The GenScript CloneEZ® PCR Cloning Kit can be used in a variety of applications:
  • PCR cloning
  • HTP PCR cloning
  • Joining of DNA fragments

    • The inserts were detected by PCR screening using single colonies. Some of the results are shown in the figure below.


    Technical Manual


    Components
    CloneEZ® Enzyme (5 U/μl) 50 μl
    10X CloneEZ® Reaction Buffer 100 μl
    pUC57 Linearized (EcoRI/HindIII,100 ng/¦Ìl) 10 μl
    1-kb Control Insert (100 ng/μl) 10 μl


    Document