High-Stability PCR Kit

GenScript High-Stability PCR Kit contains Green Taq DNA polymerase, 10× Taq buffer, and Stabilized dNTP Mix, 10 mM Each, all specially formulated for increased stability using our proprietary technology. The kit can be stored at 4℃. for over six months or at room temperature (25℃) for one month without significant loss of activity. The stabilizers in the buffers also increase the stability of Green Taq DNA polymerase at higher temperature (72℃), increasing the DNA yield of PCR amplifications of long DNA. This kit facilitates quick and easy PCR setup without any need for thawing. This kit can also be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR setup at any time.

Price	$68.00
Cat. No.	L00342
Size	1kit Kit Buy in bulk
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Product Introduction

Product Description

GenScript High-Stability PCR Kit contains Green Taq DNA polymerase, 10X reaction buffer, and 10 mM dNTP, all specially formulated for increased stability using our proprietary technology. The kit can be stored at 4°C for over six months or at room temperature (25°C) for one month without significant loss of activity. The stabilizers in the buffers also increase the stability of Taq DNA polymerase at higher temperature (72°C), increasing the DNA yield of PCR amplifications of long DNA. This kit facilitates quick and easy PCR setup without any need for thawing. This kit can also be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR setup at any time.

Key Features

High Stability: The kit remains stable for more than six months when stored at 4°C or for one month when stored at room temperature (25°C) without significant activity loss.
High PCR yield: The green Taq DNA polymerase has longer enzyme half-life and therefore increases the PCR yield when amplifying long DNA.
Terminal Transferase Activity: Taq DNA polymerase has terminal transferase activity that results in the addition of a single nucleotide (adenosine) at 3’-end of the extension product.

Storage

This product is shipped at ambient temperatures. The kit will remain stable for six months if stored at 4°C and for two years if stored at -20°C.

Kit Contents

Kit Contents	Quantity	Components/Concentration
Green Taq DNA polymerase	100 μl	5 units/μl with stabilizers
10X Reaction Buffer (with Mg²⁺)	1.5 ml	500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl₂, 1% Triton X-100 and stabilizers.
Stabilized dNTP	300 μl	10 mM with stabilizers

Green Taq DNA Polymerase and Stabilized dNTP are also available separately.
Green Taq DNA Polymerase, 100 μl (500 U), Cat. No. E00043
10X Taq Buffer (with Mg²⁺), 1.5 ml, Cat. No. B0005
Stabilized dNTP Mix, 300 μl (10 mM each), Cat. No. C01689

Back

Figure 1. A 8-kb fragment from λDNA was amplified by using both Taq DNA polymerase and green Taq DNA polymerase. For long DNA fragment amplification, where longer elongation time is needed (in this case, the elongation time was three minutes), the green Taq DNA polymerase has a longer enzyme half-life and therefore increases the PCR DNA yield significantly. »

Figure 2. A 2-kb 23S rRNA fragment from E. coli genomic DNA was amplified using green Taq DNA polymerase stored at -20°C and 37°C, respectively. The green Taq DNA polymerase can be stored at 37°C for 14 days without significant activity loss. »