Protein Detection and Immunoassay        
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L00411
1 kit (1x96 well)
$298

GST tag ELISA Detection Kit



Description:

GenScript GST tag ELISA Detection Kit is designed to quantitate glutathione-S-transferase (GST) or GST-fusion protein in samples such as cell lysate using a proprietary sandwich ELISA procedure in just 60 minutes. It utilizes two clones of GST monoclonal antibodies that bind to different epitopes of the GST tag protein at the same time. The GST monoclonal antibody, pre-coated on the plate, is used to capture GST tagged protein and the biotin conjugated GST monoclonal antibody (Biotin-GST Mab) is added to interact with GST tagged protein bound on the plate. Streptavidin-HRP is added to interact with Biotin-GST Mab and the final step involves the addition of TMB Substrate that will react with HRP to give product with an absorbance at 450 nm. The quantity of GST tagged protein in sample is determined by comparisons with the absorbance of that of a known GST tagged protein standard curve.

Key Features:
  • Time saving: This kit allows user to quantitate GST or GST-fusion protein in just 60 minutes.
  • High Sensitivity: 1 ng/ml GST tagged protein
  • Broad Detection Range: Higher specificty to detect GST protein from 3.125-100 ng/ml
  • Optimized procedure: Provide all reagents required for test
  • Highly reproducible results: Lot-to-lot consistency guaranteed by strict procedure


Applications:
  • Quantitate GST or GST-fusion proteins.
  • Check GST or GST-fusion protein expression.
  • Screen GST-fusion protein expression for expression optimization.


Storage:

  • The unopened kit is stable for at least 12 months stored at 2-8℃.
  • The opened kit may be stored for up to 1 month at 2-8°C.
  • Use only before expiration date.
  • Do not freeze the kit.

Technical Manual


Components
Pre-coated microwell plate (8 wells x 12 strips) 1
Sample Dilution solution 30 ml
Biotin-GST Mab Stock 200 µl
Antibody Dilution Buffer 15 ml
Streptavidin-HRP 12 ml
GST Protein Stock (1.0 mg/ml) 10 µl
20 x Wash solution 40 ml
TMB Substrate 12 ml


Document


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