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Cat. No.
 Name
Size   
Price
 Selected
L00419-100
 QuickClean II PCR Extraction Kit
100 rxns
$85
L00419-50
 QuickClean II PCR Extraction Kit
50 rxns
$45

QuickClean II PCR Extraction Kit


Description
The QuickClean II PCR Purification Kit provides a simple, rapid and effective method for purification of 60 bp to 10 kb DNA fragments from PCR and other enzymatic reactions. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion, PCR/real-time PCR and so on.


Key Feature
  • Easy to Perform: The QuickClean II PCR Purification Kit provides a simple, rapid and effective method for purification of 60 bp to 10 kb DNA fragments from PCR and other enzymatic reactions. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion, PCR/real-time PCR and so on.
  • High Capacity: Up to 20 µg DNA each column.
  • High Purity: The kit completely removes non-nucleic acid contaminants and inhibitors, such as agarose, proteins, salts, and ethidium bromide during washing process.


Applications
The QuickClean II PCR Purification Kit enables the fast purification of high-quality DNA products from PCR reactions and other enzymatic reactions. The extracted DNA is immediately ready for further downstream applications such as the following:
  • PCR and cloning
  • Restriction enzyme digestion
  • Transformation
  • Sequencing
  • In vitro translation


Examples
Figure 1. The following example shows the performance after impurity is removed from target DNA fragment (10 kb) using this kit according to the contrast of the gray scale.

Lane U: unpurified PCR products (10 kb)
Lane P: purified PCR products
Lane M: DL15,000 DNA Marker (Takara)

Figure 2. According to the absorbance shown is a comparison of the absorbance result before and after PCR products (1007 bp) extraction using QuickClean II PCR Extraction Kit, the DNA yield is 96% according to the contrast of gray scale.


Figure 3. Recovery of DNA is up to 97% for purification 100 bp to 10 kb DNA fragments from primers, nucleotides, polymerases, and the purification yield is up to 95% for purifying 100 bp or lower size DNA fragments.

Technical Manual


Components
Binding Buffer 20 ml
Wash Buffer 30 ml
Elution Buffer 20ml
Spin Columns 100


Document


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