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Antibody Sequencing Services
Antibody Sequencing Services

Antibody Sequencing Services

Knowing the sequence of your monoclonal antibody is not only the first step towards antibody engineering and function optimization, but also critical for patent application. GenScript's advanced technology offers fast and professional sequencing services for your hard-won monoclonal antibodies. We are capable of sequencing most antibodies with customer-selected specifications (see below for a list of different specifications). You can rely on our ISO 9001 certified Antibody Sequencing Service (Cat. No.: SC1408) to speed and facilitate your patent application, database banking, and therapeutic antibody development.

Top 3 pitfalls to avoid when sequencing antibodies

  1. Impurity of hybridoma cells: Hybridomas may contain pseudogenes and mRNAs encoding non-functional antibodies, which may be amplified by primer pairs intended to clone the target antibody.
    • GenScript will sequence 5 independent clones for each chain, to ensure accuracy.
  2. Mutations introduced by PCR: During the amplification of the cDNA, the use of degenerate primer sets means there is the chance of mutagenesis.
    • GenScript always uses both 5'- and 3'- RACE (rapid amplification of cDNA ends) to ensure accuracy of amplified products.
    • GenScript always performs full length sequencing of the variable region, which ensures the accuracy of the sequence span, from leader sequence to the start of FR1 region.
  3. Full sequence is inaccurate or misaligned: Once the individual sequences are obtained for each chain, they must be aligned properly to ensure the full sequence is accurate.
    • GenScript has sequenced over 1000 antibodies, our expertise ensures you will receive a 100% accurate sequence.
  • Service Specifications
  • Delivery
  • Technical Flow
  • Related Services

Express Sequencing Services

Sequencing Services Delivery Time * Deliverable
Variable domain & leader sequence sequencing
10 days
  • Detailed work performance report
  • Sequence data
  • Alignments of the heavy and light chains
  • Plasmid containing antibody coding sequence
Full length antibody sequencing
15 days

Standard Sequencing Services

Sequencing Services Delivery Time * Deliverable
Variable domain & leader sequence sequencing
2-3 weeks
  • Detailed work performance report
  • Sequence data
  • Alignments of the heavy and light chains
  • Plasmid containing antibody coding sequence
Full length antibody sequencing
3-4 weeks

* Our record is 6 days for full length antibody sequencing, and delivery time largely depends on the right isotype & species information provided by customers.

Customer provides

For sequencing services: You need to submit hybridoma cell samples (>1×106 cells) to GenScript.

GenScript delivers

For sequencing services: Detailed work performance report; Sequence data; Alignments of the heavy and light chains; Plasmid containing V-region sequence

The client retains all rights to the sequence data and related intellectual property.The final report includes the detailed work performance, sequence data and alignments of the heavy and light chains. If you need, we also provide the plasmid containing V-region sequence for free.

Following is GenScript's standard sequencing procedures.

mAb sequencing procedure

GenScript offers our customers a one-stop solution for all outsourcing needs, providing comprehensive upstream and downstream services of monoclonal antibody sequencing service.

 

Key Features

  • Extreme accuracy by meticulous operation and strict quality control
  • Fast delivery, starting from 6 days
  • Competitive price, starting from $1200
  • Comprehensive services covering human, mouse, rat and rabbit mAbs

Quotation and Ordering

Monoclonal Antibody Sequencing Service Quotation

  • To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.
  • To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our Secure Online Messaging System.
  • If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Antibody Drug Department, GenScript USA Inc., 860 Centennial Avenue., Piscataway, NJ08854, US.
  • For questions, or to inquire about the status of your order contact us by email, phone, fax or via our Secure Online Messaging System.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.