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Mitochondrial Apoptosis Detection Kit

Mitochondrial Apoptosis Detection Kit utilizes JC-1, a cationic dye that fluoresces differently in healthy cells and in apoptotic cells. In healthy cells, JC-1 accumulates and aggregates in the mitochondria, giving off a bright red fluorescence. In apoptotic cells, the altered potential of mitochondrial transmembrane causes JC-1 to remain in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using the FITC channel for green monomers (Ex/Em =488/530+ 30 nm) and the PI channel for red aggregates (Ex/Em =488/590+ 42 nm).

Key Features:

Extra Convenience: The kit is in ready-to-use format.
Quick Procedure: Assay can be finished in 30 minutes.
Enhanced Versatility: Directly analyzes by flow cytometer and fluorescence microscope.

Examples:

Fluorescence microscopy analyzed apoptotic and healthy cells stained with Mitochondrial Apoptosis Detection Kit (L00291). The results are as follows:

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