| Product Name |
Biotin-Protein G |
Description |
Protein G, a cell surface protein from Group G streptococci, binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. It contains three high-affinity binding sites that are capable of interacting with the Fc region of IgG in humans, rabbits, mice, cows, goats, sheep, and other species. Biotin-Protein G, which is produced by covalently linking biotin to protein G, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry. |
|
Concentration |
1 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide. |
Storage |
Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use. |
Application |
Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration.
Dot blot: 1:2,000-1:50,000
Western blot: 1:2,000-1:50,000
ELISA: 1:50,000-1:200,000
Other: user-optimized |
 |
1 |
Gene Synthesis: Custom gene synthesis service starts at as LOW as $0.35/bp, with advanced OptimumGeneTM codon optimization technology and CloneEZ® seamless cloning technology. (starting from $0.29/bp for non-codon optimized human, mouse and rat gene sequences) |
2 |
PCR Cloning and Subcloning: Order GenScript's flexible PCR cloning and subcloning service RIGHT NOW to get fully validated clones delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: Special mutagenesis bundle starts at $149/mutation. Site-directed Mutagenesis services include point mutations, deletions, and insertions for unparalleled accuracy, unlimited sites and comprehensive, fully validated deliverables. |
4 |
Plasmid DNA Preparation: Endotoxin FREE plasmid DNA preparation services deliver predominantly supercoiling plasmid DNA ranging from 100 ug to 1000 mg starting from $45. |
|
DATASHEET: 20070208032719 (PDF)