| Product Name |
HRP-Protein L |
Description |
Protein L is a cell surface protein expressed by Peptostreptoccocus magnus, that binds to the variable light chains (kappa chains) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. HRP-Protein L is produced by covalently linking HRP to protein L. The complex can be used in immuno-detection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry. |
|
Concentration |
1 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide |
Storage |
The reagent may be stored at 4°C for short periods of up to one-month. For longer periods, aliquot and store at the reagent at -20°C for longer storage. Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use. |
Application |
The ideal working concentrations for each specific application should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points:
Dot blot: 1:2,000-1:20,000
Western blot: 1:2,000-1:20,000
ELISA: 1:20,000-1:200,000
Other applications: user-optimized
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