| Product Name |
T-47D |
Figures |
Species |
Human |
References:
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Peralta EA, et al. Effect of vitamin E on tamoxifen-treated breast cancer cells. Surgery. Oct 2006; 140(4): 607-615.
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Korah R, et al. Coordinate loss of fibroblast growth factor 2 and laminin 5 expression during neoplastic progression of mammary duct epithelium. Hum. Pathol. Sep 2006.
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Zhao X, et al. Epigallocatechin gallate, the main ingredient of green tea induces apoptosis in breast cancer cells. Front Biosci. Sep 2006; 11: 2428-2433.
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Tissue |
Other |
Description |
This product consists of lysed human breast carcinoma cells. T47D cells carry receptors for a variety of steroids and calcitonins. They are responsive to estrogen and mutant tumor suppressor protein p53. Under normal culturing conditions, they express progesterone receptor constitutively. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. These cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors. 100 μg/100 μl.
|
Concentration |
Concentration: 100 μg/100 μl. Preservative: None Constituents: 20 % glycerol, 20 mM HEPES, 100 mM potassium chloride, 1mM Magnesium Chloride, 0.5 mM PMSF, and 0.5 mM DTT. pH 7.9 |
Note |
These extracts have been quality control tested by Western blot and the electrophoretic mobility shift assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
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Storage |
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
Purity |
Whole cell lysate
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Application |
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
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