DNA Marker |
Immunology Reagents |
Protein Marker |
RNA Marker |
Lysates |
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| Product Name |
293 |
Figures |
Species |
Human |
Document:
References:
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Estacion M, et al. Human TRPC6 expressed in HEK 293 cells forms non-selective cation channels with limited Ca2+ permeability. J. Physiol. Apr 2006; 572(Pt 2): 359-377.
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Tarabova B, et al. Inorganic mercury and methylmercury inhibit the Cav3.1 channel expressed in human embryonic kidney 293 cells by different mechanisms. J. Pharmacol. Exp. Ther. Apr 2006; 317(1): 418-427.
|
Tissue |
Genito-urinary |
Description |
293 whole cell lysate. |
Concentration |
Concentration: 100 μg/100 μl
Storage buffer: Whole lysate in 1 x SDS sample buffer containing 5% b-mercaptoethanol
|
Volume |
20 μg per lane. |
Storage |
The lysate may be store at -20˚C for three months or at 70˚C for one year.
|
Background |
293 cell lysate is prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris is removed by centrifugation. Protein concentration was determined via Bio-Rad protein assay. The cell lysate is boiled for five minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, and 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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