DNA Marker |
Immunology Reagents |
Protein Marker |
RNA Marker |
Lysates |
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| Product Name |
K562 |
Figures |
Species |
Mouse |
Document:
References:
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Hua JY, Huang RW. In vitro study of cytotoxic T lymphocyte activation by antigen-loaded dendritic cells for killing of K562 cells. Nan Fang Yi Ke Da Xue Xue Bao. May 2006; 26(5): 617-619.
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Schnekenburger M, et al. Transcriptional and post-transcriptional regulation of glutathione S-transferase P1 expression during butyric acid-induced differentiation of K562 cells. Leuk Res. May 2006; 30(5): 561-568.
|
Tissue |
mylogeneous leukemia |
Description |
K562 whole cell lysate |
Concentration |
Concentration: 100 μg/100 μl.
Storage buffer: whole cell lysate in 1 x SDS sample buffer containing 5% b-mercaptoethanol. |
Volume |
20 ug per lane. |
Storage |
Store at -20˚C for three months or at 70˚C for one year.
|
Background |
K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. |
Application |
K562 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 ug per lane is recommended for mini gel.
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