Custom Monoclonal Antibody Services

GUARANTEE: Two ELISA positive clones with any antigen or only $800 setup fee.

1. SC1040: Partial Monoclonal Antibody Package

Phase I: Immunization and ELISA screening. Time: 8-10 weeks.

• Conjugation: The peptide or small protein is conjugated to a carrier protein, such as KLH. Normally, 4 mg of protein immunogene or 5-10 mg of peptide is required.
• Immunization: 5 Balb/c mice are immunized and boosted with standard immunization protocol.
• Screening: Tail bleedings are screened by ELISA.

Phase II: Fusion and screening. Time: 4-8 weeks.

• Fusion: Splenocyte fusions are performed on the mouse that responded the best to the immunization.
• Expansion and screening: The hybridoma cells are expanded and screened by ELISA to obtain the highest-titer clones.

Phase III: Subcloning and delivery. Time: 4-6 weeks.

• Subcloning: Subclone two positive parental clones (up to five) by limiting dilution and isotyped.
• Delivery: One vial of cell culture for each clone and 5 ml of culture supernatants from the clones is delivered to the customer. Additional parental clones are available at an extra charge.

Total Time: 4-6 months.

2. SC1041: Complete Peptide Monoclonal Antibody Package

Phase I: Peptide synthesis ($480.00). Time: 3-4 weeks.

• 10 mg of 15 aa peptide with 90% purity is synthesized. 5 mg of free peptide will be delivered to the customer together with the antibody.

Phase II: Immunization and ELISA screening. Time: 8-10 weeks.

• Conjugation: The peptide or small protein is conjugated to a carrier protein, such as KLH. Normally, 4 mg of protein immunogene or 5-10 mg of peptide is required.
• Immunization: 5 Balb/c mice are immunized and boosted with standard immunization protocol.
• Screening: Tail bleedings are screened by ELISA.

Phase III: Fusion and screening. Time: 4-8 weeks.

• Fusion: Splenocyte fusions are performed on the mouse that responded the best to the immunization.
• Expansion and screening: The hybridoma cells are expanded and screened by ELISA to obtain the highest-titer clones.

Phase IV: Subcloning and delivery. Time: 4-6 weeks.

• Subcloning: Subclone two positive parental clones (up to five) by limiting dilution and isotyped.
• Delivery: One vial of cell culture for each clone and 5 ml of culture supernatants from the clones is delivered to the customer. Additional parental clones are available at an extra charge.

Total Time: 5-7 months.