Custom Monoclonal Antibody Services
We guarantee two positive clones for monoclonal antibodies development if peptide antigens were designed, synthesized, and conjugated by GenScript. For any protein antigen (at least 2 mg, > 50% purity) we guarantee two positive clones with positive Western blot results, or client pays only $800 set up fee.
1. SC1040: Basic Monoclonal Antibody Package (Mouse)
SC1391: Basic Monoclonal Antibody Package (Rat)
Phase I: Immunization and ELISA screening. Time: 8-10 weeks.
- Conjugation: The peptide or small protein is conjugated to a carrier protein, such as KLH. Normally, 2 mg of protein immunogen or 5-10 mg of peptide is required.
- Immunization: 5 Balb/c mice (3 rats) are immunized and boosted with standard immunization protocol.
- Screening: Tail bleedings are screened by ELISA or other method if needed.
Phase II: Fusion and screening. Time: 4-8 weeks.
- Fusion: Up to 2 fusions (1-2 spleens per fusion) are performed on the best mice that responded the best to the immunization. Additional fusions are available at an extra charge.
- Screening: The hybridoma cells are expanded and screened by ELISA or other method if needed to obtain satisfied clones.
Phase III:Subcloning, expansion, cryopreservation and delivery. Time: 4-6 weeks.
- Subcloning: Subclone up to 5 parental clones by limiting dilution and isotyped, expand and freeze 2 subclones for each parental clone.
- Delivery: Two vials of cell culture for each subclone and 5 ml of culture supernatants from the clones is delivered to the customer. Additional parental clones are available at an extra charge.
Total Time: 4-6 months.
2. SC1041: Complete Peptide Monoclonal Antibody Package (Mouse)
SC1392: Complete Peptide Monoclonal Antibody Package (Rat)
Phase I: Peptide synthesis. Time: 3-4 weeks.
- 15 mg of 15 aa peptide with 90% purity is synthesized. 5 mg of free peptide will be delivered to the customer together with the antibody.
Phase II: Immunization and ELISA screening. Time: 8-10 weeks.
- Conjugation: The peptide or small protein is conjugated to a carrier protein, such as KLH. Normally, 5-10 mg of peptide is required.
- Immunization: 5 Balb/c mice (3 rats) are immunized and boosted with standard immunization protocol.
- Screening: Tail bleedings are screened by ELISA or other method if needed.
Phase III: Fusion and screening. Time: 4-8 weeks.
- Fusion: Up to 2 fusions (1-2 spleens per fusion) are performed on the best mice that responded the best to the immunization. Additional fusions are available at an extra charge.
- Screening: The hybridoma cells are expanded and screened by ELISA or other method if needed to obtain satisfied clones.
Phase IV: Subcloning, expansion, cryopreservation and delivery. Time: 4-6 weeks.
- Subcloning: Subclone up to 5 parental clones by limiting dilution and isotyped, expand and freeze 2 subclones for each parental clone.
- Delivery: Two vials of cell culture for each subclone and 5 ml of culture supernatants from the clones is delivered to the customer. Additional parental clones are available at an extra charge.
Total Time: 5-7 months.
Phase I: Peptide synthesis. Time: 3-4 weeks.
- We will synthesize 15 mg of phospho-peptide (up to 15 residues) with >90% purity and 10 mg non-phospho-peptide with >70% purity. 5 mg of phospho-peptide and 5 mg of non-phospho-peptide will be delivered to the customer along with the final antibody product.
Phase II: Immunization and ELISA screening. Time: 8-10 weeks.
- Conjugation: The modified peptide is conjugated to a carrier protein, such as KLH.
- Immunization: 10 Balb/c mice are immunized and boosted with a standard immunization protocol.
- Screening:
Tail bleedings are screened by ELISA or other method if needed with phospho and non-phospho-peptide.
Phase III: Fusion and screening. Time: 4-8 weeks.
- Fusion: 2 fusions (1-2 spleens per fusion) are performed on the best mice that responded the best to the phospho-peptide in Phase II. Additional fusions are available at an extra charge.
- Screening: The hybridoma cells are screened by ELISA or other method if needed with phospho and non-phospho-petide and all positive clones are
expanded into 24-well plates. The clones with best ELISA value and
specificity to phospho-peptide are selected for subcloning.
Phase IV: Subcloning, expansion, cryopreservation and delivery. Time: 4-6 weeks.
- Subcloning: Hybridoma subclones are screened by ELISA with phospho
and non-phospho-petide. We subclone up to five positive parental
clones by limiting dilution, isotype the final
positive supernatants, and expand and cryopreserve two subclones
for each parental.
- Delivery: Two vials of frozen cells and 5 ml of culture supernatants for a selected subclone. We will
gladly provide subcloning for orders involving additional parental
clones for an additional fee.
Total Time: 5-7 months. Note: All antisera or purified antibodies are provided as lyophilized form except special requirements. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: antibody@genscript.com
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
 | Fax: 1-732-210-0262 1-732-885-5878 |
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