Phospho Specific Antibodies
Phosphorylation/dephosphorylation is one of the major signaling mechanisms used to modulate the functional properties of proteins involved in gene expression, cell adhesion, cell proliferation, cell differentiation, and the cell cycle. The determination of the state of phosphorylation is highly important for studying the function of a given protein. Phospho-specific antibodies, which can discriminate between the phosphorylated and non-phosphorylated forms, have emerged as demonstrated assets in the study of signal transduction pathways. These novel tools enable qualitative and quantitative detection of phosphorylated proteins without the risks associated with using radioactivity.
GenScript's phospho-specific antibodies are affinity-purified and do not cross react with non-phosphorylated forms of proteins. Phospho-specific antibodies detect only the phosphorylated forms of proteins in a complex protein mixture within cells. They recognize the phosphorylated amino acids in the context of the surrounding amino acid sequence. Using a combination of pan-antibody (that recognizes both phosphorylated and non-phosphorylated forms of the protein) and phospho-specific antibody, it is possible to assess the degree of phosphorylation for any given protein.