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Custom Polyclonal Antibody Services (Chicken)

1. SC1053: Partial Polyclonal Antibody Package (Chicken)

Includes:

  • Conjugation to KLH (optional)
  • Two chickens, pre-immune bleed, four immunizations/chicken
  • 2 ml bleed from each chicken
  • ELISA test
  • If the antigen is provided by the customer, 2 mg of peptide or protein is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.

Delivery:

  • 2 ml of pre-immune serum
  • ELISA results
  • 4 ml antiserum and 8-12 yolks from two chickens

Time Frame:

  • Conjugation: 1 week ELISA results
  • Immunization: 10-11 weeks
  • ELISA and Shipping: 1 week
  • Total 12-14 weeks

2. SC1054: Complete Peptide Polyclonal Antibody Package (Chicken)

Includes:

  • Peptide synthesis (<15 a.a., >85% purity, 10 mg)
  • A cysteine is automatically added to the C-terminus (or N-terminus) of the peptide and conjugated to KLH.
  • Two chickens, pre-immune bleed, four immunizations/chicken
  • 2 ml antiserum from each chicken
  • ELISA test

Delivery:

  • 2 ml of pre-immune serum
  • ELISA results
  • 4 ml antiserum and 8-12 yolks from two chickens
  • 2-3 mg of unconjugated peptide, MS and HPLC reports

Time Frame:

  • Peptide Synthesis: 2 weeks
  • Conjugation: 1 week
  • Immunization: 10-11 weeks
  • ELISA and Shipping: 1 week
  • Total 14-16 weeks

3. SC1055: Complete Peptide Polyclonal IgY Antibody Package (Chicken)

Includes:

  • Peptide synthesis (<15 a.a., >85% purity, 10 mg)
  • A cysteine is automatically added to the C-terminus (or N-terminus) of the peptide and conjugated to KLH.
  • Two chickens, pre-immune bleed, four immunizations/chicken
  • 4 ml antisera from two chickens
  • IgY Antibody purification
  • ELISA test

Delivery:

  • 2 ml of pre-immune serum
  • 4 ml of antiserum
  • ELISA results
  • Purified IgY antibodies
  • 2-3 mg of unconjugated peptide, MS and HPLC reports

Time Frame:

  • Peptide Synthesis: 2-4 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 15-18 weeks

4. SC1056: Complete Affinity-Purified Peptide Polyclonal Antibody Package (Chicken)

Includes:

  • Peptide synthesis (<15 a.a, >85% purity, 15 mg)
  • A cysteine is automatically added to the C-terminus (or N-terminus) of the peptide and conjugated to KLH.
  • Two chickens, pre-immune bleed, four immunizations/chicken
  • 2 ml final bleed from each chicken
  • Antibody purification by peptide affinity column from yolk
  • ELISA test

Delivery:

  • 2 ml of pre-immeune serum
  • 4 ml of antiserum
  • ELISA results
  • Affinity-purified IgY antibodies
  • 2-3 mg of unconjugated peptide, MS and HPLC reports

Time Frame:

  • Peptide Synthesis: 2-4 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 15-18 weeks

Note

If no specific request, 0.02% w/v sodium azide will be added in the antiserum and antibody products to prevent bacteria growth.