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Custom Polyclonal Antibody Services (Goat)

1. SC1052: Partial Polyclonal Antibody Production Package (Goat)

Includes:

  • Conjugation to KLH (optional)
  • Two goats, pre-immune bleed, four immunizations/goat
  • 1,100-1,200 ml of antisera from both goats
  • ELISA test
  • If the antigen is provided by the customer, 5 mg of peptide or protein is required. The concentration should be >0.4 mg/ml, and the buffer must not contain organic solvents.

Delivery:

  • 2-5 ml of pre-immune sera
  • ELISA results
  • 1,100-1,200 ml of antisera from two goats

Time Frame:

  • Conjugation: 1 week
  • Immunization: 10 weeks
  • ELISA and Shipping: 1-2 weeks
  • Total 12-13 weeks

2. SC1050: Complete Peptide Polyclonal Antibody Package (Goat)

Includes:

  • Peptide synthesis (<15 a.a, >85% purity, 10 mg)
  • A cysteine is automatically added to the C-terminus (or N-terminus) of the peptide and conjugated to KLH.
  • Two goats, pre-immune bleed, four immunizations/goat
  • 1,000-1,200 ml final bleed from both goats
  • ELISA test

Delivery:

  • 2-5 ml of pre-immune sera
  • ELISA results
  • 1,100-1,200 ml of antisera from both goats
  • 5 mg of unconjugated peptide, MS and HPLC reports

Time Frame:

  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • ELISA and Shipping: 1-2 weeks
  • Total 14-16 weeks

3. SC1051: Complete Protein G-Purified Peptide Polyclonal Antibody Package (Goat)

Includes:

  • Peptide synthesis (<15 a.a, >85% purity, 10 mg)
  • A cysteine is automatically added to the C-terminus (or N-terminus) of the peptide and conjugated to KLH.
  • Two goats, pre-immune bleed, four immunizations/goat
  • 500ml-600 ml antiserum from each goat
  • Protein G purification of 100 ml antisera
  • ELISA test

Delivery:

  • 2-5 ml of pre-immune sera
  • ELISA results
  • Purified G-purified antibody from 100 ml antisera
  • ≈1000 ml antisera
  • 5 mg of unconjugated peptide, MS and HPLC reports

Time Frame:

  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 15-17 weeks

Note

If no specific request, 0.02% w/v sodium azide will be added in the antierum and antibody products to prevent bacteria growth.