Custom Polyclonal Antibody Services (Goat)
1. SC1052: Partial Polyclonal Antibody Production Package (Goat)
Includes:
- Conjugation to KLH (optional)
- Two goats, pre-immune bleed, four immunizations/goat
- 1,100-1,200 ml of antisera from both goats
- ELISA test
- If the antigen is provided by the customer, 5 mg of peptide or protein is required. The concentration should be >0.4 mg/ml, and the buffer must not contain organic solvents.
Delivery:
- 2-5 ml of pre-immune sera
- ELISA results
- 1,100-1,200 ml of antisera from two goats
Time Frame:
- Conjugation: 1 week
- Immunization: 10 weeks
- ELISA and Shipping: 1-2 weeks
- Total 12-13 weeks
2. SC1050: Complete Peptide Polyclonal Antibody Package (Goat)
Includes:
- Peptide synthesis (<15 a.a, >85% purity, 10 mg)
- A cysteine is automatically added to the C-terminus (or N-terminus) of the peptide and conjugated to KLH.
- Two goats, pre-immune bleed, four immunizations/goat
- 1,000-1,200 ml final bleed from both goats
- ELISA test
Delivery:
- 2-5 ml of pre-immune sera
- ELISA results
- 1,100-1,200 ml of antisera from both goats
- 5 mg of unconjugated peptide, MS and HPLC reports
Time Frame:
- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- ELISA and Shipping: 1-2 weeks
- Total 14-16 weeks
3. SC1051: Complete Protein G-Purified Peptide Polyclonal Antibody Package (Goat)
Includes:
- Peptide synthesis (<15 a.a, >85% purity, 10 mg)
- A cysteine is automatically added to the C-terminus (or N-terminus) of the peptide and conjugated to KLH.
- Two goats, pre-immune bleed, four immunizations/goat
- 500ml-600 ml antiserum from each goat
- Protein G purification of 100 ml antisera
- ELISA test
Delivery:
- 2-5 ml of pre-immune sera
- ELISA results
- Purified G-purified antibody from 100 ml antisera
- ≈1000 ml antisera
- 5 mg of unconjugated peptide, MS and HPLC reports
Time Frame:
- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification: 1 week
- ELISA and Shipping: 1-2 weeks
- Total 15-17 weeks
Note
If no specific request, 0.02% w/v sodium azide will be added in the antierum and antibody products to prevent bacteria growth.
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