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Custom Polyclonal Antibody Services (Mouse)

1. SC1047: Partial Polyclonal Antibody Package (Mouse)

Includes:

  • Conjugation to KLH (optional)
  • Five Balb/c mice, pre-immune bleed, four immunizations/mouse
  • 2.5 ml of antiserum from all five mice
  • ELISA test
  • Antigens provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>75% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.

Delivery:

  • 50-100 µl of pre-immune serum
  • ELISA results
  • 2.5 ml of antiserum from all mice

Time Frame:

  • Conjugation: 1 week
  • Immunization: 10 weeks
  • ELISA and Shipping: 1-2 weeks
  • Total 12-13 weeks

2. SC1250: Partial Protein A-Purified Polyclonal Antibody Package (Mouse)

Includes:

  • Conjugation to KLH (optional)
  • 5 Balb/c mice, pre-immune bleed, 4 immunizations/mouse
  • Final bleed
  • Protein A purification
  • ELISA test
  • Antigen provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>75% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.

Delivery:

  • 50-100 µl of pre-immune serum
  • ELISA results
  • Protein A-purified antibodies
  • Remained antigen

Time Frame:

  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Protein A Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 13-14 weeks

3. SC1046: Complete Peptide Polyclonal Antibody Package (Mouse)

Includes:

  • Peptide synthesis (<15 a.a, >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Five Balb/c mice, pre-immune bleed, four immunizations/mouse
  • 2.5 ml of antiserum from all mice
  • ELISA test

Delivery:

  • 50-100 µl of pre-immune serum
  • ELISA results
  • 2.5 ml of antiserum from all mice
  • 5 mg of unconjugated peptide, MS and HPLC report

Time Frame:

  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • ELISA and Shipping: 1-2 weeks
  • Total 14-16 weeks

4. SC1049: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse)

Includes:

  • Peptide synthesis (<15 a.a, >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Five Balb/C mice, pre-immune, four immunizations/mouse
  • 2.5 ml of antiserum from all mice
  • Antibody purification by protein A column
  • ELISA test

Delivery:

  • 50-100 µl of pre-immune serum
  • ELISA results
  • Purified antibody from all antiserum
  • 5 mg of unconjugated peptide, MS and HPLC report

Time Frame:

  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 15-17 weeks

Note

If no specific request, 0.02% w/v sodium azide will be added in the antiserum and antibody products to prevent bacteria growth.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Email: antibody@genscript.com
Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Fax: 1-732-210-0262 1-732-885-5878
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