Custom Polyclonal Antibody Services (Mouse)
1. SC1047: Basic Polyclonal Antibody Package (Mouse)
Includes |
- Conjugation to KLH (optional)
- Five Balb/c mice, pre-immune bleed, four immunizations/mouse
- ELISA test
- Lyophilization
- Antigens provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.
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Delivery |
- 50-100 µl of pre-immune serum (lyophilized)
- ELISA results
- 2.5 ml of antisera (lyophilized)
from all mice
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Time frame |
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 12-13 weeks
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2. SC1250: Basic Protein A-Purified Polyclonal Antibody Package (Mouse)
Includes |
- Conjugation to KLH (optional)
- 5 Balb/c mice, pre-immune bleed, 4 immunizations/mouse
- Final bleed
- Protein A purification
- ELISA test
- Lyophilization
- Antigen provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.
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Delivery |
- 50-100 µl of pre-immune serum (lyophilized)
- ELISA results
- 2.5-12.5 mg protein A-purified antibodies (lyophilized)
- Remained antigen
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Time frame |
- Conjugation: 1 week
- Immunization: 10 weeks
- Protein A Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 13-14 weeks
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3. SC1046: Complete Peptide Polyclonal Antibody Package (Mouse)
Includes |
- Peptide synthesis (<15 a.a, >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/c mice, pre-immune bleed, four immunizations/mouse
- ELISA test
- Lyophilization
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Delivery |
- 50-100 µl of pre-immune serum (lyophilized)
- ELISA results
- 2.5 ml of antisera (lyophilized) from all mice
- 5 mg of unconjugated peptide, MS and HPLC report
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Time frame |
- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 14-16 weeks
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4. SC1049: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse)
Includes |
- Peptide synthesis (<15 a.a, >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/C mice, pre-immune, four immunizations/mouse
- Antibody purification by protein A column
- ELISA test
- Lyophilization
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Delivery |
- 50-100 µl of pre-immune serum (lyophilized)
- ELISA results
- 2.5-12.5 mg purified antibody (lyophilized) from all antisera
- 5 mg of unconjugated peptide, MS and HPLC report
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Time frame |
- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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Note
If no specific request, 0.02% w/v sodium azide will be added in the antisera
and antibody products to prevent bacteria growth. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped. All antisera or purified antibodies are provided as lyophilized form except special requirements.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: antibody@genscript.com
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
 | Fax: 1-732-210-0262 1-732-885-5878 |
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