Custom Polyclonal Antibody Services (Rabbit) |
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1. SC1030: Basic Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Conjugation to KLH (optional)
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- ELISA test
- Lyophilization
- If the antigen is provided by the customer: 2.5-3 mg of peptide or 2.5-3 mg of protein is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.
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- 2 ml of pre-immune serum (lyophilized)
- ELISA results
- 100-120 ml of antisera (lyophilized) from two rabbits
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- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 12-13 weeks
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2. SC1247: Basic Antigen Affinity-Purified Polyclonal Antibody Package (Rabbit)
Service Package |
Peptide Antigen |
Protein Antigen |
| Includes |
- Conjugation to KLH
- 2 rabbits, pre-immune bleed, 4 immunizations/rabbit
- Final bleed
- Peptide conjugated to affinity resin
- Affinity purification of antibodies
- ELISA test
- Lyophilization
- Antigen provided by the customer: 7.5-8 mg of peptide (2.5-3 mg for immunization, 5 mg for purification)
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- Protein checking by SDS-PAGE
- 2 rabbits, pre-immune bleed, 4 immunizations/rabbit
- Final bleed
- Protein conjugated to affinity resin (Dialyze the protein with PBS if needed)
- Affinity purification of antibodies
- ELISA test
- Lyophilization
- Antigen provided by the customer: 7.5-8 mg of protein (2.5-3 mg for immunization, 5 mg for purification).The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution. Tris and other solution containing amino groups should not be used.
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| Delivery |
- 1-2 ml of pre-immune serum (lyophilized)*
- ELISA results
- 3-5 mg affinity-purified antibodies (lyophilized)
- Remaining antigen
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- 1-2 ml of pre-immune serum (lyophilized)*
- ELISA results
- 3-5 mg affinity-purified antibodies (lyophilized)
- Remaining antigen
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| Time Frame |
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 13-14 weeks
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- Protein checking: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 13-14 weeks
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3. SC1248: Basic Protein A-Purified Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Conjugation to KLH (optional)
- 2 rabbits, pre-immune bleed, 4 immunizations/rabbit
- Final bleed
- Protein A purification
- ELISA test
- Lyophilization
- Antigen provided by the customer: 2.5-3 mg of peptide or 2.5-3 mg of protein is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.
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- 1-2 ml of pre-immune serum (lyophilized)*
- ELISA results
- 200-500 mg protein A-purified antibodies (lyophilized)
- Remaining antigen
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- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 13-14 weeks
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4. SC1015: Complete Peptide Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- ELISA test
- Lyophilization
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- 2 ml of pre-immune serum (lyophilized)
- ELISA results
- 100-120 ml of antisera (lyophilized) from two rabbits
- 5mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 14-16 weeks
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5. SC1045: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune, four immunizations/rabbit
- Final bleed
- Protein A purification
- ELISA test
- Lyophilization
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- 1-2 ml of pre-immune serum (lyophilized)*
- ELISA results
- 200-500 mg protein A-purified antibodies (lyophilized)
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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6. SC1031: Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- Peptide (5 mg) conjugated to affinity resin
- Affinity purified antibodies
- ELISA test
- Lyophilization
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- 1-2 ml of pre-immune serum (lyophilized)*
- ELISA results
- 3-5 mg affinity-purified antibodies (lyophilized)
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ESLIA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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7. SC1044: Phospho-Specific Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Synthesis of two peptides, one with a modified amino acid (<15 a.a, >90% purity, 15 mg)and the other without modified amino acid (<15 a.a, >90% purity, 10 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Prepare two affinity columns
- Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
- ELISA test
- Lyophilization
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- 1-2 ml of pre-immune serum (lyophilized)*
- ELISA results
- 1-3 mg modified-specific antibodies (lyophilized) from two rabbits
- 5 mg of modified peptide and 5 mg of unmodified-peptide, MS and HPLC reports
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- Peptide Synthesis: 2-4 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-18 weeks
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* For collection and purification of antibodies from separated
animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2
animals) are provided. For antibody purification from
pooled serum, 1 ml of pre-immune serum is provided.
Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera or antibody products to prevent bacteria growth. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped. All antisera or purified antibodies are provided as lyophilized form except special requirements.
Quotation and Ordering:
 To request a quotation, please download and complete our Quick Quotation Request. |
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| To order a pAb, please download & complete our Polyclonal Antibody Order Form. |
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| To submit an antigen, mail it together with a completed hard copy of Antigen Submission |
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| Form to Antibody Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US. |
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Please email or fax all quotation requests or completed order forms (including the Antigen Submission Forms) to GenScript.
If you have any questions or would like to inquire about the status of your order, please contact us by phone, email, fax, or via our Secure Web Server.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: antibody@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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