||rHuBMP-2; Bone Morphogenetic Protein2; BMP-2; BMP2
||Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and a member of the transforming growth factor-β (TGF-β) superfamily of proteins. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or as a heterodimer of two related bone morphogenetic proteins.
Recombinant human Bone Morphogenetic Protein-2 (BMP-2) produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 26,018 Da. Human Bone Morphogenetic Protein-2 (BMP-2) is purified by proprietary chromatographic techniques.
||Recombinant human Bone Morphogenetic Protein-2 (BMP-2) is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml corresponding to a specific activity of 2.0 x 104 IU/mg.
||The sequence of the first five N-terminal amino acids has been found to be Met-Gln-Ala-Lys-His.
||The protein was lyophilized after extensive dialysis against 50mM Acetic acid.
||It is recommended that the lyophilized rHuBMP-2 be reconstituted in sterile 20 mM HAc (acetic acid) not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
||Greater than 95.0% as determined by the following methods:
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
||Less than 0.1 ng/μg (1 IEU/μg) of Bone Morphogenetic Protein-2
||Lyophilized recombinant human Bone Morphogenetic Protein-2 (BMP-2) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution rHuBMP-2 should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.
Zhao M, et al. The zinc finger transcription factor gli2 mediates bone morphogenetic protein 2 expression in osteoblasts in response to hedgehog signaling. Mol. Cell Biol. Aug 2006; 26(16): 6197-6208.
Hoshino M, et al. Repair of long intercalated rib defects using porous beta-tricalcium phosphate cylinders containing recombinant human bone morphogenetic protein-2 in dogs. Biomaterials. Oct 2006; 27(28): 4934-4940.