Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), human
| Full Name |
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Human
|
Abbreviated Name-1 |
rHuGM-CSF; GM-CSF |
|
Description |
Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils.
Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. |
Source |
E. coli |
M.W. |
14,000 Da |
UV Scan |
The maximal absorption wave is 279+/-3 nm. |
Purity |
Greater than 95% as determined by
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel |
Endotoxin Level |
Less than 0.03 ng/µg (0.3 IEU/μg) determined by LAL test |
Specific Activity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0×107 IU/mg. |
Storage |
Lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF), although stable at room temperature for three weeks, should be stored desiccated at -18°C or below. Upon reconstitution, Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) may be stored at 4°C for short periods (two to seven days). For long term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles. |
Formulation |
The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.0, 150mM NaCl, 5% Mannitol buffer. |
Reconstitution |
It is recommended that the lyophilized rHuGM-CSF be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Sequence |
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE |
Sequence analysis |
The sequence of the first fifteen N-terminal amino acids has been found to be (Met)-Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr-Gln-Pro-Trp-Glu-His.
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Note |
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1 |
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2 |
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3 |
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4 |
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5 |
Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level. |
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Document-MSDS: 11008_20091015014536.PDF (PDF)