IFN-α 2b, Interferon-α 2b, human
| Full Name |
IFN-α 2b, Interferon-α 2b, Human
|
Abbreviated Name-1 |
rHuIFN-α-2b; rHuIFNα-2b; rHuIFN-a-2b; rHuIFN-alfa-2b; rHuIFNalfa-2b; Interferon-α-2b; Interferonα-2b; Interferon-a-2b; Interferon-alfa-2b; Interferonalfa-2b |
| Documents |
|---|
 Document-MSDS: 15076_20111227042733.PDF (PDF) |
| Document-COA: 15486_20120206005420.PDF (PDF) |
| Figures |
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| Reference |
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Siebeck N, et al. Effects of human recombinant alpha-2b interferon and feline recombinant omega interferon on in vitro replication of feline herpesvirus-1. Am. J. Vet. Res. Aug 2006; 67 (8): 1406-1411.
Abukawa H, et al. Effect of interferon-alpha-2b on porcine mesenchymal stem cells. J. Oral Maxillofac. Surg. Aug 2006; 64 (8): 1214-1220.
Yang L, et al. A novel method to prepare highly encapsulated interferon-alpha-2b containing liposomes for intramuscular sustained release. Eur. J. Pharm. Biopharm. Aug 2006; 64 (1): 9-15.
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Description |
Human Interferon alpha 2b (IFN α2b) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Da. The Interferon alpha 2b (IFN α2b) gene was obtained from human leukocytes. The Recombinant Human Interferon alpha 2b (IFN α2b) is purified by proprietary chromatographic techniques. |
Source |
E. coli |
M.W. |
19,269 Da |
UV Scan |
The maximal absorption wave is 278+/-3 nm. |
Purity |
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Endotoxin Level |
Less than 0.1 ng/µg (IEU/µg) of rHuIFN-α 2b |
Specific Activity |
The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6×108/mg. |
Storage |
Lyophilized Recombinant Human Interferon alpha 2b (IFN α2b) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human Interferon alpha 2b (IFN α2b) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer. |
Reconstitution |
It is recommended to reconstitute the lyophilized rHuIFN-α 2b in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Quantitation |
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2b as a Reference Standard. |
Sequence analysis |
The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln. N-terminal methionine has been completely removed enzymatically.
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Note |
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