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Z00358-1	IFN-α 2a, Interferon-α 2a, human	1 mg	$990
Z00358-10	IFN-α 2a, Interferon-α 2a, human	10 μg	$35
Z00358-50	IFN-α 2a, Interferon-α 2a, human	50 μg	$120

IFN-α 2a, Interferon-α 2a, human

Full Name

IFN-α 2a, Interferon-α 2a, Human

Abbreviated Name-1

rHuIFN-α 2a; rHuIFNα 2a; rHuIFN-a 2a; rHuIFN-alfa 2a; rHuIFNalfa 2a; Interferon-α 2a; Interferonα 2a; Interferon-a 2a; Interferon-alfa 2a; Interferonalfa 2a

Documents
Document-MSDS: 15096_20111227201925.PDF (PDF)
Document-COA: 15488_20120206010938.PDF (PDF)
Figures
Reference
Jo YW, et al. Long-acting interferon-alpha 2a modified with a trimer-structured polyethylene glycol: preparation, in vitro bioactivity, in vivo stability and pharmacokinetics. Int. J. Pharm. Feb 2006; 309 (1-2): 87-93. Asik Akman S, et al. Preliminary results of interferon alpha-2a and lamivudine combination therapy regimen in children with chronic hepatitis B. Mikrobiyol. Bul. Jan-Apr 2006; 40 (1-2): 47-53. Ajariyakhajorn C, et al. Randomized, placebo-controlled trial of nonpegylated and pegylated forms of recombinant human alpha interferon 2a for suppression of dengue virus viremia in rhesus monkeys. Antimicrob. Agents Chemother. Nov 2005; 49 (11): 4508-4514.

Description

Human Interferon alpha 2a (IFN alpha 2a) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,241 Da. The Interferon alpha 2a (IFN alpha 2a) gene was obtained from human leukocytes. The Recombinant Human Interferon alpha 2a (IFN alpha 2a) is purified by proprietary chromatographic techniques.

Source

E. coli

M.W.

19,241 Da

Purity

Greater than 98.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Endotoxin Level

Less than 0.1 ng/µg (IEU/µg) of rHuIFN-α 2a

Specific Activity

The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1.0 x 10⁸ IU/ mg.

Storage

Lyophilized Recombinant Human Interferon alpha 2a (IFN alpha 2a) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution recombinant Human Interferon alpha 2a (IFN alpha 2a) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.

Formulation

Lyophilized from (1 mg/ml) solution containing 7.21 sodium chloride and 0.77 mg ammonium acetate.

Reconstitution

It is recommended to reconstitute the lyophilized rHuIFN-α 2a in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Quantitation

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-α 2a as a Reference Standard.

Sequence analysis

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-α. N-terminal methionine has been completely removed enzymatically.

Note

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