Products
24 hour
You are here: Products » Recombinant Proteins » Enterokinase, Light Chain, Porcine
GenScript Share
 
Search by Catalog Number :
Search by Name :
Search by Sequence : (One letter code)
Search by Category :





Cat. No.
 Name
Size   
Price
 Selected
Z01003
100 IU
$80.00

Full Name Enterokinase, Light Chain, Porcine
Abbreviated Name-1
 Enterokinase; EK
Documents
Enterokinase, Light Chain, PorcineDocument-MSDS: 1447_20060502233525.PDF (PDF)
Enterokinase, Light Chain, PorcineDocument-EXAMPLE: 1643_20060705015011.JPG (JPG)
Enterokinase, Light Chain, PorcineTECHNICAL MANUAL: 2444_20061122042342.PDF (PDF)
Enterokinase, Light Chain, PorcineDocument-COA: 11726_20100125024403.PDF (PDF)
Enterokinase, Light Chain, PorcineDocument-COA: 11904_20100220214710.PDF (PDF)
Enterokinase, Light Chain, PorcineDocument-COA: 12262_20100310220154.PDF (PDF)
Enterokinase, Light Chain, PorcineDocument-COA: 14297_20111114035438.PDF (PDF)
Enterokinase, Light Chain, PorcineDocument-COA: 14298_20111114035438.PDF (PDF)
Figures
Reference
Palmai-Pallag T, et al. The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins. FEBS J. Jun 2005; 272(11): 2901-2911.

Liew OW, et al. Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites. Protein Expr. Purif. Jun 2005; 41(2): 332-340.

Peng L, et al. High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris. J. Biotechnol. Mar 2004; 108(2): 185-192.
Description
 GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.
Source
 Yeast
M.W.
 Theoretical MW: 21,880 Da
The apparent MW on SDS-PAGE: about 40,000 Da
Specific Activity
 One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.
Storage
 Store at -20°C after delivery.
Formulation
 GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.
Concentration
 50 ul, 2 U/ul
1
 Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2
 Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3
 Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
4
 Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5
 Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.