| Full Name |
Proteinase K
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Abbreviated Name-1 |
Proteinase K; |
| Documents |
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 Document-MSDS: 15509_20120216222411.PDF (PDF) |
| Figures |
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| Reference |
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Gao JM, et al. Influence of guanidine on proteinase K resistance in vitro and infectivity of scrapie prion protein PrP(Sc). Acta. Virol. 2006; 50(1): 25-32.
Sabarth N, et al. Identification of Helicobacter pylori surface proteins by selective proteinase K digestion and antibody phage display. J. Microbiol. Methods. Sep 2005; 62(3): 345-359.
Yamashita K, et al. Enzymatic degradation of poly(L-lactide) film by proteinase K: quartz crystal microbalance and atomic force microscopy study. Biomacromolecules. Mar-Apr 2005; 6(2): 850-857.
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Description |
Protease K is a subtilian-type protease isolated from the saprophytic fugus Tritirachim album. It is particularly suitable for short digestions. It possesses a very broad peptide cleavage activity, which remains stable over a wide range of temperatures and pH values, although its activity increases at higher temperature. |
Source |
Tritirachim album |
Specific Activity |
>30 mAU/mg. Unit Definition: One mAU is the amount of proteinase that can release folin-positive amino acids and peptides corresponding to 1 µmol tyrosine per minute. Recommended Reaction Buffer: 50 mM Tris-HCl (pH=7.5), 10 mM CaCl2. No RNase and DNase activity detected. |
Storage |
Store at -20°C. |
Applications |
Genomic DNA purificationRemoval enzymes in DNA and RNA preparationPreparation of chromosomal DNA for pulsed-field gel electrophoresis
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Note |
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