| Full Name |
Protein G
|
Abbreviated Name-1 |
Protein G |
| Documents |
|---|
 COA: Z02007_P01120601 (PDF) |
| Figures |
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zoom |
| Reference |
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Kang M, et al. G-protein coupled receptor signaling in myocardium: not for the faint of heart. Physiology (Bethesda). Jun 2007; 22(3): 174-184.
Nitsche-Schmitz DP, et al. Group G Streptococcal IgG Binding Molecules FOG and Protein G Have Different Impacts on Opsonization by C1q. J. Biol. Chem. Jun 2007; 282(24): 17530-17536.
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|
Abbreviated Name-2 |
G Protein |
Description |
Protein G, a cell surface protein of group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A from Staphylococcus aureus. This product contains three IgG binding domains, but its albumin-binding region has been removed. Additionally, His-tag and cysteine have been added to N- and C-termini, respectively, to facilitate purification and conjugation. This does not affect the protein's ability to bind to IgG. |
Source |
Streptococcus G148 gene expression in E. coli |
M.W. |
29,000-35,000 Da was determined by SDS-PAGE while relative molecular mass calculated from amino acid sequence is ≈24,000 Da. |
Purity |
This product is chromatographically purified and shows predominantly a single band by SDS-PAGE.
Focusing: pI of 4.69 |
Specific Activity |
The theoretical binding capacity is 3 mol IgG/mol protein G. Protein G is assayed using ELISA. The optimal concentration for coating plate is 5 ug/ml. |
Storage |
This product is lyophilized in 1X PBS. It will remain stable at 4°C for several weeks. For longer periods, store it at -20°C. |
Concentration |
5 mg/ml before lyophilization |
Applications |
GenScript Protein G is suitable for use as antigen, as a control or standard in assays, for conjugation, and most other immunological methods. |
