| Full Name |
Epstein-Barr Virus (HHV-4) Mosaic EBNA2 Protein, Recombinant
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Abbreviated Name-1 |
EBV (HHV-4) mosaic EBNA1; |
| Documents |
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| Figures |
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| Reference |
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Zhang D, et al. Human RPA (hSSB) interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus.Nucleic Acids Res. Jan 1998; 26(2): 631-637.
Cruickshank J, et al. Two domains of the epstein-barr virus origin DNA-binding protein, EBNA1, orchestrate sequence-specific DNA binding. J. Biol. Chem. Jul 2000; 275(29): 22273-22277.
Kapoor P, Frappier L. EBNA1 partitions Epstein-Barr virus plasmids in yeast cells by attaching to human EBNA1-binding protein 2 on mitotic chromosomes. J. Virol. 2003 Jun; 77(12): 6946-6956.
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Description |
The protein is E. coli-derived recombinant. The mosaic protein contains the HHV-4 EBNA regions. The EBNA1 (Epstein-Barr nuclear antigen 1) protein of the Epstein-Barr virus governs the replication and partitioning of the viral genomes during latent infection by binding to specific recognition sites in the viral origin of DNA replication. |
Purity |
Protein is >95% pure as determined by 10% PAGE (Coomassie staining). |
Storage |
The protein is shipped at ambient temperature. Upon arrival, store it at -20°C. |
Concentration |
1 mg/ml in 50 mM Tris-HCl, 10 mM glutation, 60 mM NaCl, 0.5% sarcosyl |
Stability |
The protein will remain stable for five years if stored frozen and for one month if stored in solution at room temperature. |
Specificity |
The protein is immunoreactive with the sera of EBV-infected individuals. |
Applications |
The protein makes a good antigen in ELISA and western blots and an excellent antigen in the detection of HHV-4 (EBV). It shows minimal specificity problems. |
Note |
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