| Full Name |
Interleukin-8 (IL-8) (8-79), Human
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Abbreviated Name-1 |
Interleukin (IL)-8 (8-79) |
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Description |
Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.
GenScript Interleukin (IL)-8 (8-79), produced in E. coli is a single, non-glycosylated polypeptide chain containing 72 amino acids and having a molecular mass of 8,452 Da. |
M.W. |
8,000 Da+/-10% determined by reduced SDS-PAGE |
Purity |
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE silver stained gel |
Endotoxin Level |
Less than 0.3 ng/µg (0.3 IEU/µg) determined by LAL test |
Storage |
Lyophilized rHuIL-8 /72 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-8 /72 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Formulation |
Lyophilized from a concentrated (1 mg/ml) solution containing 20 mM PBS pH-7.4 and 50 mM sodium chloride. |
Reconstitution |
It is recommended to reconstitute the lyophilized rHuIL-8 /72 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Specificity |
rHuIL-8 /72 is fully biologically active when compared to standard. The ED50 as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells is less then 0.5 ng/ml, corresponding to a specific activity of 2.0×106 IU/mg. |
Sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Lys-Glu-Leu. |
