| Full Name |
Endostatin, Human
|
Abbreviated Name-1 |
Endostatin; |
|
Description |
Endostatin is a cleaved product of the carboxyl-terminal domain of collagen XVIII. It functions as an anti-angiogenic cytokine that is expressed in various organs especially with high levels in liver, lung, and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant Endostatin expressed in yeast, whichcauses G1 arrest of endothelial cells. Endostatin treatment results in apoptosis of HUVE and HMVE cells. Recombinant human Endostatin is a 20.2 kDa protein consisting of 184 amino acid residues. |
Source |
P. Pichia |
Purity |
Greater than 97% by SDS-PAGE |
Endotoxin Level |
Endotoxin level is less than 0.1 ng per μg (1EU/μg) of Endostatin. |
Specific Activity |
Determined by Endothelial Cell Tube Formation. The cells treated with 10μg/ml Endostatin showed significant anti-angiogenesis effect. |
Storage |
The lyophilized Endostatin is stable for a few weeks at room temperature, but best stored at -20°C. For the first use, reconstitute in water, aliquot and store at -20°C to avoid multiple freeze-thaws. |
Formulation |
The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2. |
Document-COA: 12637_20100419060450.PDF (PDF)