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Interleukin-17F (IL-17F), Human



Cat. No.
Name
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Selected
Z02713-1
1 mg
$2,100
Z02713-25
25 μg
$159

Protein Interleukin-17F (IL-17F), Human
Synonyms
Interleukin-17F (IL-17F), Human;
Documents
IL-17F, HumanDocument-MSDS: 15168_20111231003258.PDF (PDF)
Species
Human
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 1 EU/μg of rHuIL-17F as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg.
Source
E. coli
Amino Acid Sequence
MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS
MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN
SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ
Molecular Weight
Approximately 30.1 kDa, a disulfide-linked homodimer of two 134 amino acid polypeptide chains.
Description
Human IL-17F is synthesized as a 153 aa precursor with a 20 aa signal sequence and a 133 aa mature region. Like IL-17A, IL-17F contains one potential site for N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence identity. IL17-F homodimer is produced by an activated subset of CD4+ T cells, termed Th17. IL17-F has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Document-COA
  • Z02713_R50291204.pdf
  • Z02713_R51821204.pdf
  • Citation
    Kim G, Khanal .et al Interleukin-17 induces AP-1 activity and cellular transformation via upregulation of tumor progression locus 2 activity. Carcinogenesis. 2013 Feb;34(2):341-50.

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