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Interleukin-17F (IL-17F), Human

Synonyms Interleukin-17F (IL-17F), Human;
Description Human IL-17F is synthesized as a 153 aa precursor with a 20 aa signal sequence and a 133 aa mature region. Like IL-17A, IL-17F contains one potential site for N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence identity. IL17-F homodimer is produced by an activated subset of CD4+ T cells, termed Th17. IL17-F has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.
Amino Acid Sequence
Source E. coli
Species Human
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Molecular Weight Approximately 30.1 kDa, a disulfide-linked homodimer of two 134 amino acid polypeptide chains.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with trehalose.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Endotoxin Level Less than 1 EU/μg of rHuIL-17F as determined by LAL method.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

Kim G, Khanal .et al Interleukin-17 induces AP-1 activity and cellular transformation via upregulation of tumor progression locus 2 activity. Carcinogenesis. 2013 Feb;34(2):341-50.

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Cat. No. Z02713
25 ug $159.00
1 mg $2100.00