Catalog Products » Recombinant Proteins » Interleukin-17F (IL-17F), Human
 
Search by Catalog Number :
Search by Name :
Search by Sequence : (One letter code)
Search by Category :



Interleukin-17F (IL-17F), Human



Cat. No.
Name
Size   
Price
Selected
Z02713-1
1 mg
$2,100
Z02713-25
25 μg
$159

Full Name Interleukin-17F (IL-17F), Human
Abbreviated Name-1
Interleukin-17F (IL-17F), Human;
Documents
Interleukin-17F (IL-17F), HumanDocument-MSDS: 15168_20111231003258.PDF (PDF)
Description
Human IL-17F is synthesized as a 153 aa precursor with a 20 aa signal sequence and a 133 aa mature region. Like IL-17A, IL-17F contains one potential site for N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence identity. IL17-F homodimer is produced by an activated subset of CD4+ T cells, termed Th17. IL17-F has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.
Source
Escherichia coli.
Molecular Weight
A disulfide-linked homodimer of 30.1kDa, consisting of two 134 amino acid polypeptide chains.
Purity
>95% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rHuIL-17F as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 15 ng/ml, corresponding to a specific activity of > 6.7 × 104 IU/mg.
Storage
This lyophilized preparation is stable for several weeks at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Amino Acid Sequence
MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS
MSRNIESRSTSPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN
SVPIQQETLVVRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ
Document-COA
  • Z02713_R50291204.pdf
  • Z02713_R51821204.pdf
  • Citation
    Kim G, Khanal .et al Interleukin-17 induces AP-1 activity and cellular transformation via upregulation of tumor progression locus 2 activity. Carcinogenesis. 2013 Feb;34(2):341-50.

    1
    Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
    2
    Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
    3
    Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
    4
    Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
    5
    Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.