| Full Name |
Interleukin-17F (IL-17F), Human
|
Abbreviated Name-1 |
Interleukin-17F (IL-17F), Human; |
|
Description |
Human IL-17F is synthesized as a 153 aa precursor with a 20 aa signal sequence and a 133 aa mature region. Like IL-17A, IL-17F contains one potential site for N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence identity. IL17-F homodimer is produced by an activated subset of CD4+ T cells, termed Th17. IL17-F has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis. |
Source |
Escherichia coli. |
M.W. |
A disulfide-linked homodimer of 30.1kDa, consisting of two 134 amino acid polypeptide chains. |
Purity |
>95% by SDS-PAGE and HPLC analyses. |
Endotoxin Level |
Less than 0.2EU/ug of rHuIL-17F as determined by LAL method. |
Specific Activity |
Fully biologically active when compared to standard. The ED50 determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 15 ng/ml, corresponding to a specific activity of ＞ 6.7 × 104 IU/mg. |
Storage |
This lyophilized preparation is stable for several weeks at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
Formulation |
Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Usage |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Sequence |
MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRSTSPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLVVRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ |
Document-MSDS: 15168_20111231003258.PDF (PDF)