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TARC/CCL17, Human



Cat. No.
Name
Size   
Price
Selected
Z02840-1
1 mg
$2,400
Z02840-20
20 μg
$157

Full Name TARC/CCL17, Human
Abbreviated Name-1
TARC/CCL17, Human;
Documents
TARC/CCL17, HumanDocument-MSDS: 15380_20120105040009.PDF (PDF)
TARC/CCL17, HumanDocument-COA: R51221204_Z02840_COA.pdf (pdf)
Figures
Reference
Description
CCL17 is a novel CC chemokine recently identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1ª, MIP-1ß, MCP-1, MCP-2, MCP-3 and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was recently identified to be a specific functional ligand for CCR-4, a receptor that is selectively expressed on T cells.
Source
Escherichia coli.
M.W.
8.0kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.
Purity
>97% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rHuTARC/CCL17 as determined by LAL method.
Specific Activity
Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Storage
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Sequence

ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV
FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S
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