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MIP-4/CCL18, Human



Cat. No.
Name
Size   
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Selected
Z02841-1
1 mg
$2,100
Z02841-10
10 μg
$159

Full Name MIP-4/CCL18, Human
Abbreviated Name-1
MIP-4/CCL18, Human;
Documents
MIP-4/CCL18, HumanDocument-MSDS: 15382_20120105040207.PDF (PDF)
Description
CCL18, is a novel CC chemokine that is highly homologous to MIP-1ª (61% amino acid sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-γ. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.
Source
E. coli
Molecular Weight
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 1 EU/μg of rHuMIP-4/CCL18 as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml.
Storage
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Amino Acid Sequence
AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI
LLTKRGRQIC ADPNKKWVQK YISDLKLNA
Document-COA
  • R51231204_Z02841_COA.pdf
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