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MIP-4/CCL18, Human


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Cat. No.
Product Name
Size
Price
Quantity
Z02841-10 MIP-4/CCL18, Human
10 μg
$159.00
Z02841-1 MIP-4/CCL18, Human
1 mg
$2100.00

 


Synonyms MIP-4/CCL18, Human;
Description CCL18, is a novel CC chemokine that is highly homologous to MIP-1ª (61% amino acid sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-γ. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.
Amino Acid Sequence
AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI
LLTKRGRQIC ADPNKKWVQK YISDLKLNA
Source E. coli
Species Human
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Molecular Weight Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Endotoxin Level Less than 1 EU/μg of rHuMIP-4/CCL18 as determined by LAL method.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

MIP-4/CCL18, Human

MIP-4/CCL18, Human

MIP-4/CCL18, Human




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