Bone Morphogenetic Protein-2 (BMP-2), Human- GenScript, Your Reliable Source for Functional Proteins

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Full Name

Bone Morphogenetic Protein-2 (BMP-2), Human

Abbreviated Name-1

BMP-2, BMP2A, Bone morphogenetic protein 2, BMP-2A, BMP2

Documents
Document-COA: COA of Human BMP-2.pdf (pdf)
Document-MSDS: MSDS of Human BMP-2.pdf (pdf)
Figures
Reference
Sampath, T.K. et al., Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily. J Biol Chem. 1990 Aug 5;265(22):13198-205. Heng S. et al., Posttranslational activation of bone morphogenetic protein 2 is mediated by proprotein convertase 6 during decidualization for pregnancy establishment. Endocrinology. 2010 Aug;151(8):3909-17. Epub 2010 Jun 16. Celeste, A. J. et al., Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9843-7.

Description

Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met¹-Arg³⁹⁶, with a signal peptide from Met¹ to Gly²³), propeptide (Leu²⁴-Arg²⁸²) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys³⁶⁰. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction.

Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 115 amino acids. A fully biologically active molecule, rhBMP-2 has a molecular mass of 26 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Source

E. coli

M.W.

26 kDa, observed by non-reducing SDS-PAGE

Purity

> 95% as analyzed by non-reducing SDS-PAGE.

Endotoxin Level

< 1 EU/μg, determined by LAL method.

Specific Activity

ED₅₀ < 1 μg/ml, measured by alkaline phosphatase induction assay using C2C12 cells.

Storage

Lyophilized recombinant human Bone Morphogenetic Protein-2 (BMP-2) remains stable at room temperature for two weeks. Upon reconstitution, rhBMP-2 should be stable up to 1 month at 4°C and up to 3 months at -20°C.

Formulation

Lyophilized after extensive dialysis against 50 mM acetic acid.

Reconstitution

Reconstituted in 20 mM AcOH/5 mM HCl. The solubility should be at 100 μg/ml.

Sequence

Gln²⁸³-Arg³⁹⁶ (accession number:P12643). Expressed with an N-terminal Met.

Note


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