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Leptin, Human



Cat. No.
Name
Size   
Price
Selected
Z02962-1
1 mg
$159

Protein Leptin, Human
Synonyms
Obesity protein (OB)
Documents
Leptin, HumanDocument-MSDS: Z02962_MSDS.pdf (pdf)
Species
Human
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 1 EU/μg of rHuLeptin as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Source
E. coli
Amino Acid Sequence
VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD
FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL
AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL
DLSPGC
Molecular Weight
Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Document-COA
  • C50611303_Z02962_COA.pdf
  • C54531302_Z02962_COA.pdf
  • C59691302_Z02962_COA.pdf
  • R50051507_Z02962_COA.pdf
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