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Interleukin-1 beta (IL-1β), Rat



Cat. No.
Name
Size   
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Z03014-1
1 mg
$2,100

Protein Interleukin-1 beta (IL-1β), Rat
Synonyms
IL1F2, IL1B, Catabolin, Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
Documents
IL-1β, RatDocument-MSDS: Z03014_MSDS.pdf (pdf)
Species
Rat
Purity
> 95% by SDS-PAGE and HPLC analyses.
Endotoxin Level
< 0.2 EU/μg, determined by LAL method.
Biological Activity
ED50 < 10 pg/ml, measured by a cell proliferation assay using mouse D10S cells, corresponding to a specific activity of > 1.0× 108 units/mg.
Source
E. coli
Amino Acid Sequence
Val117-Ser268 (accession number: Q63264).
Molecular Weight
17.3 kDa, observed by reducing SDS-PAGE.
Description
Interleukin-1 Beta (IL-1 Beta) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.

Recombinant rat Interleukin-1 Beta (rat IL-1 Beta) produced in E. coli is a single non-glycosylated polypeptide chain containing 152 amino acids. A fully biologically active molecule, recombinant rat IL-1 Beta protein has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Formulation
Lyophilized after extensive dialysis against PBS.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Storage
Lyophilized recombinant rat Interleukin-1 Beta (IL-1 Beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant rat IL-1 Beta should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Document-COA
  • P50011302_Z03014_COA.pdf
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