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6Ckine/CCL21, Rat

*This product has been discontinued!*
Recombinant rat 6Ckine/CCL21 produced in CHO cells is a polypeptide chain containing 120 amino acids. A fully biologically active molecule, rr6Ckine/CCL21 has a molecular mass of 12 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03325
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Product Introduction
Species Rat
Protein Construction
CCL21 (Ser24-Gln133)
Accession # P02775
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity The EC50 value of Rat 6Ckine/CCL21 on Ca2+ mobilization assay in CHO-K1/G15/h_CCR7 pool (human G15 and human CCR7 stably expressed in CHO-K1 cells) is less than 1.0 μg/ml.
Expression System CHO
Apparent Molecular Weight ~12 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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Background
Target Background Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine, exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 contains four conserved cysteines characteristic of β chemokines plus two additional cysteines in an unusually long carboxyl-terminal domain. It is expressed in lymphatic endothelial cells and the spleen. CCL21 chemoattracts T and B lymphocytes and inhibits hematopoiesis. It can signal through the CCR7 receptor.
Synonyms 6Ckine; exodus-2; SCYA21; SLC; TCA-4
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.