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PD-1 Fc Chimera, Human

Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279) or PDCD1, is a protein that in humans is encoded by the PDCD1 gene. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells.PD-1 binds two ligands, PD-L1 and PD-L2. PD-1 and its ligands play an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells (suppressor T cells)
$120.00
Z03370-50

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Product Introduction
Species Human
Protein Construction
PD-1 (Leu25-Gln167)
Accession # Q15116		 hFc
N-term C-term
Purity > 98% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity Assay #1: Immobilized PD-L1, hFc, Human (Cat. No.: Z03371) at 1.0 μg/ml (100 μl/well) can bind biotinylated PD-1, hFc, Human.
Assay #2: Immobilized PD-L1, His, Human(Cat. No.: Z03425) at 2.0 μg/ml (100 µl/well) can bind PD-1, hFc, Human.
Expression System CHO
Apparent Molecular Weight 60~65 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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PD-1 Fc Chimera, Human

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PD-1 Fc Chimera, Human

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PD-1 Fc Chimera, Human

Immobilized PD-L1-His,Human(Cat.No.Z03425) at 2 μg/mL (100 μL/well) can bind PD-1, Fc,Humanwith a linear range of 0.024-0.39μg/mL. »

PD-1 Fc Chimera, Human

GenScript product showed equal activity compared to competitor A. »

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Background
Target Background Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279) or PDCD1, is a protein that in humans is encoded by the PDCD1 gene. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells.PD-1 binds two ligands, PD-L1 and PD-L2. PD-1 and its ligands play an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells (suppressor T cells)
Synonyms PD-1; CD279; PDCD1; Programmed cell death 1
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Chuang Ma, et al. A Novel Type of PD-L1 Inhibitor rU1 snRNPA From Human-Derived Protein Scaffolds Library. Front Oncol. (2021-11)
Chenglong Liu, et al. Discovery of a novel, potent and selective small-molecule inhibitor of PD-1/PD-L1 interaction with robust in vivo anti-tumour efficacy. Br J Pharmacol. (2021-03)