B7-H3 (4Ig) /B7-H3b[Biotin], His & Avi, Human

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Price	$650.00
Cat. No.	Z03890-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

B7-H3 (4Ig) /B7-H3b (Gly27-Thr461) Accession # Q5ZPR3-1	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

49.5 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Anti-B7-H3 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for B7-H3 (4Ig) /B7-H3b[Biotin], His & Avi, Human with the EC50 of 7.7 ng/ml determined by ELISA. »

The purity of B7-H3 (4Ig) /B7-H3b[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

B7-H3 (4Ig) /B7-H3b[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Synonyms	B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.