VEGF R3/FLT4[Biotin], His&Avi, Human

Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.

Price	$650.00
Cat. No.	Z03971-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

VEGF R3/FLT4 (Tyr25-Ile776) Accession # P35916-1	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

87.4 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized Human VEGF-C, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for VEGF R3/FLT4[Biotin], His & Avi, Human with the EC50 of 23.0 ng/ml determined by ELISA. »

VEGF R3/FLT4[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

The purity of VEGF R3/FLT4[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

Background

Target Background	Vascular endothelial growth factor receptor 3 (VEGFR3) is one kind of tyrosine-protein kinase. VEGFR3 acts as a cell-surface receptor for VEGFC and VEGFD. It is a key regulator of lymphatic system development and establishment. VEGFR3 plays important roles in angiogenesis. It is also up-regulated in the endothelium of blood vessels in breast cancer and various other tumors.
Synonyms	VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.