CD3E/CD3 epsilon hFc Chimera, Cynomolgus

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Price	$360.00
Cat. No.	Z04011-100
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Product Introduction Background Bottom Notes

Product Introduction

Species

Cynomolgus

Protein Construction

CD3E/CD3 epsilon (Gln22-Asp117) Accession # Q95LI5-1	hFc
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

36.9 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized CD3E/CD3 epsilon hFc Chimera, Cynomolgus, hFc Tag at 2 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 14.9 ng/ml determined by ELISA. »

The purity of CD3E/CD3 epsilon hFc Chimera, Cynomolgus is greater than 95% as determined by SEC-HPLC. »

CD3E/CD3 epsilon hFc Chimera, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Background

Target Background	CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Synonyms	CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.