CD6, His, Human

The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.

Price	$340.00
Cat. No.	Z04027-100
Size	100 μg 500 μg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

CD6 (His18-Leu402) Accession # NP_006716.3	His
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

42.14 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

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Immobilized CD6, His, Human, His Tag at 1 μg/ml (100 μg/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA. »

The purity of CD6, His, Human is greater than 95% as determined by SEC-HPLC. »

CD6, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind CD6, His, Human, His Tag with an affinity constant of 0.53 μM as determined in SPR assay (Biacore T200). »

Background

Target Background	The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.
Synonyms	CD6; TP120; T12

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.